Journal:

Article Title: Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34

doi: 10.1182/blood-2004-09-3652

Figure Lengend Snippet: Cell development in short-term culture assays. ALDHneg CD34+, ALDHbr CD34+, and ALDHbr CD34neg cells were purified from linneg SSClo UCB, as depicted in Figure 1. Two hundred to 1000 purified cells were cultured on STO fibroblasts in the presence of IL-3, IL-7, and IL-15 (n = 10). Cultures initiated with ALDHneg CD34+ cells contained higher percentages of CD56+ lymphoid progeny than did the ALDHbr CD34+ cells (compare panels A and B; see Table 4). Similarly, the ALDHneg CD34+ cell fraction yielded lower percentages of CD13+ myeloid progeny (compare panels D and E). Only 2 cultures initiated with ALDHbr CD34neg cells had growth significant enough to evaluate lineage development, and these gave rise to CD56+ progeny (C,F). The CD56+ progeny of ALDHneg CD34+ (G-I) and ALDHbr CD34+ cells (data not shown) expressed other antigens consistent with NK cells. (J) Relative output of lymphoid cells was higher in cultures initiated with ALDHneg CD34+ cells when compared with paired cultures initiated with ALDHbr CD34+ cells. (K) Conversely, the relative cell output of myeloid cells was higher in cultures initiated with ALDHbr CD34+ cells when compared with paired cultures initiated with ALDHneg CD34+ cells. Estimations for relative cell output are described in “Materials and methods.” (J-K) Each data point represents an average from duplicate cultures.

Article Snippet: Short-term culture assays for lymphoid development were monitored on STO murine embryonic fibroblasts (CRL-1503; American Type Culture Collection, Manassas, VA).

Techniques: Purification, Cell Culture

Journal: bioRxiv

Article Title: True-to-scale DNA-density maps correlate with major accessibility differences between active and inactive chromatin

doi: 10.1101/2022.03.23.485308

Figure Lengend Snippet: High-resolution, true-to-scale DNA density landscape of human fibroblast nucleus a Voronoi-tessellated SMLM-fBALM image of a mid-optical section (thickness ∼100 nm through a diploid human fibroblast cell nucleus (BJ1) stained with Sytox Orange (see Methods for further explanation how this image was generated). The color code indicates the estimated range of 3D DNA densities from <5 Mbp/µm 3 to >40 Mbp/µm 3 , reflecting 2D SMP densities <2,500 SMPs/µm 2 to >20,000 SMPs/µm 2 (see and Results for 3D DNA densities estimated from 2D SMP densities in 100 nm thick sections). b, 1-3 Magnifications of the three boxed areas in (a) demonstrate sites located at the nuclear periphery (1) and in the nuclear interior (2, 3); edge length 3000 nm. Asterisks denote IC- lacunae. b, 1’–3’ Further magnification of boxed areas in (1-3), edge lengths 1000 nm (1’), and 500 nm (2’,3’). c, 1’’–3’’ upper row: Further magnification of boxed areas in (1’-3’), edge length 200 nm. lower row: Individual Voronoi centers (seeds) are shown for the same Voronoi areas as in 1’’ – 3’’. d, 1’’’–3’’’ same areas as in c 1’’ depicted here with another color code (shown at bottom) for 3D DNA densities from 40 Mbp/µm 3 to about 350 Mbp/µm 3 , reflecting a range of 2D SMP densities between ∼20,000 and ∼180,000 SMPs/µm 2 .

Article Snippet: h-TERT BJ1 cells, immortalized, diploid human cell line from human foreskin fibroblasts (ATCC #CRL-2522), HeLa cells, a human cervix carcinoma derived cell line with a near-triploid karyotype (n = 68-70 ), and C3H 10T1/2 cells, derived from murine embryo fibroblast cell line (ATCC #CCL-226) were cultured in DMEM with 5% CO2 at 37 °C.

Techniques: Staining, Generated

Journal: bioRxiv

Article Title: True-to-scale DNA-density maps correlate with major accessibility differences between active and inactive chromatin

doi: 10.1101/2022.03.23.485308

Figure Lengend Snippet: Comparison of high-resolution DNA landscapes of mouse C3H T1/2 fibroblast nuclei recorded with SMLM and ESI a 100 nm optical mid-section through a mouse C3H T1/2 fibroblast nucleus recorded with SMLM-fBALM. b-e ESI micrographs taken from acrylic 100 nm thin mid-sections through another C3H T1/2 fibroblast nucleus. b and d show superimposed elemental maps of phosphorus (green) and nitrogen (red). c and e present the phosphorus map only, color-coded for different phosphorus densities as a reflection of DNA densities. For better comparison, the same color-coded look-up table (jet) was used for SMLM (a) and ESI images (c,e; blue indicates lowest DNA densities that belong to the IC, lined by light blue and green colored intermediate densities (PR); red marks high DNA compaction attributed to the INC (compare and ). Arrows in (d) and (e) indicate examples of chromocenters. In order to secure that only the DNA landscape was recorded, RNA was digested in both nuclei prior to imaging.

Article Snippet: h-TERT BJ1 cells, immortalized, diploid human cell line from human foreskin fibroblasts (ATCC #CRL-2522), HeLa cells, a human cervix carcinoma derived cell line with a near-triploid karyotype (n = 68-70 ), and C3H 10T1/2 cells, derived from murine embryo fibroblast cell line (ATCC #CCL-226) were cultured in DMEM with 5% CO2 at 37 °C.

Techniques: Comparison, Imaging